Polymer LB films consisting of lysine and alanine derivative polymers were prepared to investigate the interfacial recognition between protein and ligand on the LB film. The surface of lysine derivative polymer LB film was modified by the biotin molecule that is the ligand for avidin. The biotinylation was carried out without inducing non-specific adsorption of proteins; avidin specifically adsorbed onto the biotinylated surfaces. Moreover, it was found that there is an optimum molar ratio of biotinylation for the adsorption of avidin onto the surface.
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Ide et al. (2001) studied this question.
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