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A split-sample experiment involving 14,319 first inseminations was carried out at sixteen artificial insemination centres to compare the fertility of semen diluted with skim-milk and skim-milk containing 10% glycerol when used on the first, second, third and fourth days after the day of collections. The addition of glycerol to the heat-treated skimmilk diluent significantly reduced the decline in fertility with increasing age. However, there were indications that glycerolization also reduced initial fertility.
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O'Connor et al. (1959) studied this question.
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