The effect of various diluents and different dilution rates on the maintenance of motility of ram sperm during storage at 4° C. was tested. Their efficiency was determined by half-life periods and total survival times of the sperm. A yolk-glycine-citrate diluent maintained sperm motility for a longer period than did yolk—fructose—phosphate, yolk-citrate, cow's milk and ewe's milk, with or without yolk. This diluent has the advantage that the sperm is clearly visible under the microscope, and no sedimentation of any component occurs. A modified IVT diluent prolonged the total life span of the sperm, but caused a faster motility decline in the first part of the storage period.
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Schindler et al. (1961) studied this question.
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