The nitrogenase components of Anabaena variabilis were isolated to prepare a specific antiserum against the cyanobacterial Fe protein. Thereby, the status of the Fe protein subunits could be analyzed after in-vivo inactivation of nitrogenase. Firstly, nitrogenase activity was switched off by adding ammonia to intact filaments accompanied with the appearance of a slower migrating subunit of the Fe protein as shown by immunospecific Western blots. Inactivation of nitrogenase was correlated with about equal amounts of modified and non-modified subunits of the Fe protein. Secondly, a rapid inactivation of nitrogenase was achieved by treatment of cells with oxygen, which was followed by a slow appearance of the modified subunit of the Fe protein.
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Reich et al. (1989) studied this question.
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