Somatic polyploidy is associated with the upregulation of c-MYC interacting genes and EMT-like signature

// Alejandro Vazquez-Martin 1, * , Olga V. Anatskaya 2, * , Alessandro Giuliani 3 , Jekaterina Erenpreisa 1, ** , Sui Huang 4 , Kristine Salmina 1 , Inna Inashkina 1 , Anda Huna 1 , Nikolai N. Nikolsky 2 , Alexander E. Vinogradov 2, ** 1 Latvian Biomedical Research and Study Centre, Riga, Latvia 2 Institute of Cytology, St-Petersburg, Russian Federation, Russia 3 Istituto Superiore di Sanità, Rome, Italy 4 Systems Biology Institute, Seattle, USA * Equal contribution ** Senior authors Correspondence to: Olga V. Anatskaya, email: olga.anatskaya@gmail.com Keywords: c-MYC interacting genes, polyploidy, Warburg, stress, EMT Received: June 03, 2016 Accepted: September 05, 2016 Published: September 19, 2016 ABSTRACT The dependence of cancer on overexpressed c-MYC and its predisposition for polyploidy represents a double puzzle. We address this conundrum by cross-species transcription analysis of c-MYC interacting genes in polyploid vs. diploid tissues and cells, including human vs. mouse heart, mouse vs. human liver and purified 4n vs. 2n mouse decidua cells. Gene-by-gene transcriptome comparison and principal component analysis indicated that c-MYC interactants are significantly overrepresented among ploidy-associated genes. Protein interaction networks and gene module analysis revealed that the most upregulated genes relate to growth, stress response, proliferation, stemness and unicellularity, as well as to the pathways of cancer supported by MAPK and RAS coordinated pathways. A surprising feature was the up-regulation of epithelial-mesenchymal transition (EMT) modules embodied by the N-cadherin pathway and EMT regulators from SNAIL and TWIST families. Metabolic pathway analysis also revealed the EMT-linked features, such as global proteome remodeling, oxidative stress, DNA repair and Warburg-like energy metabolism. Genes associated with apoptosis, immunity, energy demand and tumour suppression were mostly down-regulated. Noteworthy, despite the association between polyploidy and ample features of cancer, polyploidy does not trigger it. Possibly it occurs because normal polyploidy does not go that far in embryonalisation and linked genome destabilisation. In general, the analysis of polyploid transcriptome explained the evolutionary relation of c-MYC and polyploidy to cancer.