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Germination of rape (Brassica napus L.) seed exposed to ethyl methanesulfonate (EMS) for 12 hr at 20–25 C was differentially affected by EMS concentration, pH of treatment solution, seed size, and strain treated. All first order interactions among treatments, except EMS concentration × strain, significantly affected germination. Increasing EMS concentration from 0 to 1.0% adversely affected germination, plant vigor, and seed yield and increased the number of abnormal plants and the days to maturity in M1 populations. The types of plant abnormalities observed following EMS treatment are described.
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Fowler et al. (1972) studied this question.
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