The first sex pheromone of a Sphinx moth has been isolated from Manduca sexta (Lepidoptera: Sphingidae) with the aid of an electroantennogram assay. It is attractive to males in a field test where its activity might be augmented by a second component in female extracts. Chemical tests and chromatographic properties identify the pheromone as a C16-aldehyde with a pair of conjugated double bonds. (E , Z)-0,12-hexadecadienal, (“bombykal”) was identical with the natural product. The comparison includes chromatography on three different capillary columns and determination of specific activities in the electroantennogram test.
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Starratt et al. (1979) studied this question.