Phenotypic and Functional Characterization of In Vivo Tissue Engineered Smooth Muscle From Normal and Pathological Bladders

No AccessJournal of UrologyAugmentation/Reconstruction1 Oct 2002Phenotypic and Functional Characterization of In Vivo Tissue Engineered Smooth Muscle From Normal and Pathological Bladders Jin-Yao Lai, Cheol Yong Yoon, James J. Yoo, Tina Wulf, and Anthony Atala Jin-Yao LaiJin-Yao Lai , Cheol Yong YoonCheol Yong Yoon , James J. YooJames J. Yoo , Tina WulfTina Wulf , and Anthony AtalaAnthony Atala View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64429-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The engineering of bladder tissue involves obtaining a biopsy from a host, expanding the cells, seeding them onto a matrix and implanting the cell-matrix composite back into the host. Clinically, cells used for these techniques may be harvested from abnormal bladders. It is not known whether abnormal bladder cells may be engineered into functionally normal tissue. We investigated the phenotypic and functional characteristics of tissue engineered bladder smooth muscle derived from patients with functionally normal bladders and functionally abnormal exstrophic and neuropathic bladders. Materials and Methods: Human smooth muscle cells derived from functionally normal bladders, exstrophic bladders and neurogenic bladders were grown, expanded and seeded onto polymer scaffolds. Sixteen cell seeded scaffolds were analyzed in vitro and 40 cell seeded scaffolds were implanted in athymic mice. The tissue engineered constructs were retrieved and analyzed at 2 weeks and 2 months. The scaffolds were evaluated immunocytochemically, histologically, with organ bath studies and with Western blot analyses. Results: Human bladder cells showed similar expression of smooth muscle marker proteins (α-actin and myosin) in vitro and after 2 months in vivo, regardless of their origin. All scaffolds showed similar muscle formation in vivo. The cell seeded scaffolds demonstrated the typical "contraction-relaxation" response to supramaximal electrical field and carbachol stimulation. There were no statistical differences among the experimental groups (normal, exstrophic, neurogenic). Conclusions: Tissue engineered muscle from normal and diseased bladders retain their phenotype in vitro and after implantation in vivo. The cells exhibited the same degree of contractility to electrical and chemical stimulation regardless of their origin. These results suggest that there are no phenotypic or functional differences between muscle cells obtained from urodynamically normal or pathological bladders, and that bladder muscle cells, regardless of their origin, may have the potential to be engineered into normal bladder tissues. References 1 : The effect of gastric augmentation on bladder function. J Urol1993; 149: 1099. Link, Google Scholar 2 : Continent urinary diversion: the Children's Hospital experience. J Urol1997; 157: 1394. 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Link, Google Scholar From the Laboratory for Cellular Therapeutics and Tissue Engineering, Department of Urology, Children's Hospital and Harvard Medical School, Boston, Massachusetts© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySuson K, Stec A, Gearhart J and Shimoda L (2012) Transforming Growth Factor-β1 Mediates Migration in Cultured Human Control and Exstrophy Bladder Smooth Muscle CellsJournal of Urology, VOL. 188, NO. 4S, (1528-1534), Online publication date: 1-Oct-2012.Suson K, Stec A, Shimoda L and Gearhart J (2012) Initial Characterization of Exstrophy Bladder Smooth Muscle Cells in CultureJournal of Urology, VOL. 188, NO. 4S, (1521-1527), Online publication date: 1-Oct-2012.Kwon T, Yoo J and Atala A (2008) Local and Systemic Effects of a Tissue Engineered Neobladder in a Canine Cystoplasty ModelJournal of Urology, VOL. 179, NO. 5, (2035-2041), Online publication date: 1-May-2008.BORER J, GARGOLLO P, KINNAMON D, BAUER S, KHOSHBIN S, HENDREN W, PETERS C, DIAMOND D, ATALA A, CHIN S and RETIK A (2018) BLADDER GROWTH AND DEVELOPMENT AFTER COMPLETE PRIMARY REPAIR OF BLADDER EXSTROPHY IN THE NEWBORN WITH COMPARISON TO STAGED APPROACHJournal of Urology, VOL. 174, NO. 4 Part 2, (1553-1558), Online publication date: 1-Oct-2005. Volume 168Issue 4 Part 2October 2002Page: 1853-1858 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsbathselectric stimulationbladderpolyglycolic acidtissuesMetricsAuthor Information Jin-Yao Lai More articles by this author Cheol Yong Yoon More articles by this author James J. Yoo More articles by this author Tina Wulf More articles by this author Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...