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The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.
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Parthasarathy et al. (2010) studied this question.
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