Characterization of human sperm antigens reacting with antisperm antibodies from autologous sera and seminal plasma: comparison among infertile subpopulations

Immunoblotting techniques were used to characterize the reactivity of human sperm antigens with sperm antibodies from different groups of infertile subjects. Sperm antigens of each subject were tested with autologous sperm antibodies present in serum and seminal plasma in order to assess whether there were qualitative differences in the antigenic structure of spermatozoa among the various infertile groups. Sixty-one infertile males, divided into six groups according to changes in the two main semen parameters (sperm concentration and motility), entered the study. Immunoreactivity to several antigens, in both serum and seminal plasma, was usually demonstrated in infertile subjects. Using a complex statistical package no significant difference was found in the distribution of antigens between groups; this included previously termed "fertility-related antigens' (28, 35, 57 and 62kDa proteins). This suggests that the most relevant sperm antigens involved in reproduction have no particular relationship to sperm concentration and motility.