Key result
The 18-nucleotide region immediately 5' to the branch site imposes near maximal ATP dependence for U2 snRNP addition to pre-mRNA, independent of specific sequence or nucleobases.
Population
in vitro RNA substrates and nuclear extract
Comparison
modifying the intron region 5' to the branch site vs minimal intron-derived substrates that form…
Design
Preclinical
Authors
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No immediate clinical impact; extends spliceosome models and leaves open cardiac disease relevance.
Identifies the specific 18-nt pre-mRNA region 5' to the branch site responsible for the ATP requirement during U2 snRNP addition in spliceosome assembly.
Newnham et al. (2001) studied this question. Intron region 5' to the branch site vs. ATP-independent minimal intron-derived substrates was evaluated on Efficiency of complex formation with U2 snRNP. The 18-nucleotide region immediately 5' to the branch site imposes near maximal ATP dependence for U2 snRNP addition to pre-mRNA, independent of specific sequence or nucleobases.
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