An analytical method for the neonicotinoid pesticide nitenpyram and its metabolites CPMA and CPMF was investigated. Agricultural products were extracted with acetone followed by re-extraction using an Extrelut® column and purification by silica gel column chromatography. CPMA and CPMF were so unstable that they were derived to a more stable compound, CPF, prior to re-extraction. It was necessary to remove acetone from the extract before reaction of CPMF, because a by-product was found and the recovery of CPF decreased in the presence of acetone. Nitenpyram was determined using HPLC, and CPF, using GC-FTD. Recoveries from fruits, vegetables, rice and tea were 66-85% for nitenpyram (0.2-0.8ppm added), and 64-120% for metabolites (0.5-2.0ppm added), except for CPMA in tea. Detection limits were 0.0025-0.01ppm for nitenpyram, and 0.025-0.1ppm for metabolites.
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Tsumura et al. (1998) studied this question.