Key result
This review summarizes the replication cycle of rotavirus and the development of reverse genetics systems for double-stranded RNA viruses.
This review summarizes the development of reverse genetics systems for double-stranded RNA viruses, particularly rotavirus, providing a background for molecular studies of viral replication.
No immediate clinical applications; leaves open translation of reverse genetics systems to rotavirus therapeutics.
The rotavirus genome is composed of 11 segments of double-stranded (ds)RNA. Recent studies have elucidated the precise mechanisms in transcription and replication of rotavirus RNA mainly by in vitro experiments. However, the ideal methodology for the molecular study of rotavirus replication is reverse genetics, which enables the viral genome to be artifically manipulated. Since the development of the first reverse genetics system for RNA virus in bacteriophage QB in 1978, the methodology has been developed for a variety of RNA viruses with plus-strand, minus-strand or dsRNA as a genome. However, there have been no reports on the reverse genetics of the viruses in the family Reoviridae with a genome of 10–12 segmented dsRNA, except for reovirus. This review describes the replication cycle of rotavirus with the aim of providing a general background to the development of rotavirus reverse genetics, and summarizes the reverse genetics system for dsRNA viruses, including rotavirus.
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Komoto et al. (2006) conducted a review in Rotavirus and other segmented double-stranded RNA viruses. Reverse genetics systems was evaluated. This review summarizes the replication cycle of rotavirus and the development of reverse genetics systems for double-stranded RNA viruses.
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