Key result
Deletion or complete substitution of the UCUAAAC promoter motif in bovine coronavirus defective interfering RNA did not prevent rapid leader conversion, indicating it is not required for this event.
The UCUAAAC promoter motif is not required for leader conversion on the bovine coronavirus DI RNA 5' terminus, suggesting recombination occurs via a different mechanism than leader-primed transcription.
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Questions motif dependence for leader conversion in coronavirus DI RNA; leaves open alternative recombination mechanisms.
Chang et al. (1996) studied Bovine coronavirus infection (in vitro). Deletion or substitution of the UCUAAAC promoter motif vs. Wild-type BCV DI RNA was evaluated on Leader conversion/recombination. Deletion or complete substitution of the UCUAAAC promoter motif in bovine coronavirus defective interfering RNA did not prevent rapid leader conversion, indicating it is not required for this event.
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