Expression analysis of plectin isoforms in osteoclast

Bone homeostasis is maintained by osteoclasts and osteoblasts. Regulation of bone homeostasis is important for orthodontic treatments, oral implant, treatment for periodontal disease, osteonecrosis of the jaw and so on. We reported an intermediate filament linking protein Plectin plays important role in osteoclastic bone resorption. Plectin is a large molecule >500 kDa and have 13 isoforms. Individual Plectin isoforms have different roles. To reveal the roles of Plectin in osteoclasts, we examined the expression levels of Plectin isoforms and found the isoforms regulating actin organization are highly expressed in osteoclasts.