Separation and determination of isoflavones in the protein concentrate from red clover leaves

Oestrogenic isoflavones were extracted from the protein concentrate of red clover leaves, isolated on columns of Sephadex G‐25 using 0.1 M‐ammonia and determined spectrophotometrically in the eluted fractions. Most of the chlorophyll and its breakdown products were precipitated from the original aqueous ethanolic extract before the lipids and then the isoflavones were finally extracted.