We report an amplified chemiluminescence (CL) biosensing platform for ultrasensitive DNA detection. It is based on exonuclease III-assisted target recycling amplification and the super quenching efficiency of graphene oxide (GO). In the presence of target DNA, the target–probe hybrid forms a double-stranded structure, and exonuclease III catalyzes the stepwise removal of mononucleotides from the fluorescein-labeled probe DNA, resulting in the recycling of the target DNA and CL signal amplification of the luminol–H2O2–HRP–fluorescein chemiluminescence resonance energy transfer (CRET) system. The detection limit of target DNA was estimated to be as low as 9 fM, and the sensitivity was about 3 orders of magnitude better than that of GO-based fluorescence resonance energy transfer (FRET) sensors for DNA with exonuclease III-assisted amplification. This CL biosensor offers the advantages of being facile, sensitive, rapid and cost-effective.
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Chen et al. (2013) studied this question.
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