SUMMARY Mutants ofAspergillus flavusimpaired in aflatoxin production were induced withN-methyl-N′-nitrosoguanidine and analysed by means of the parasexual cycle. The gene symbolaflwas assigned to this type of mutation. Diploid complementation tests revealed that most of 14aflmutants belonged to different complementation groups. One mutant (afl-1) failed to complement or only partially complemented all other mutants. Haploidization of one diploid revealed the independent segregation of twoaflmutants. Heterozygous diploids were synthesized between twoaflmutants and tester strains genetically labelled on eight linkage groups. Haploidization of the diploids led to the assignment of two aft mutants to linkage groups. Linkage ofafl-4 tow+andafl-1 toleuon linkage groups II and VII, respectively, was demonstrated.
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K. E. Papa (1979) studied this question.
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