Three disulfide linkages of recombinant human brain‐derived neurotrophic factor (BDNF) were determined by peptide sequence analysis and characterized by mass spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster ovary cells include Cys‐13‐Cys‐80, Cys‐58‐Cys‐109 and Cys‐68‐Cys‐111, and the disulfide structure was homologous to that of nerve growth factor.
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Acklin et al. (1993) studied this question.
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