Key result
Single guide RNA engineering and design strategies facilitate more efficient, specific, and safer gene editing within the CRISPR/Cas system.
Why the study?
While CRISPR derivative methods are accessible, new users face an overwhelming abundance of choices, and guide RNA engineering strategies needed consolidation.
This review provides a beginner's guide to single guide RNA engineering and design strategies for the CRISPR/Cas system to facilitate efficient and safe gene editing.
Answers are grounded in this paper and cite their sources.
May aid novice CRISPR researchers; leaves cardiovascular gene-editing applications open pending validation.
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a state-of-the-art tool for versatile genome editing that has advanced basic research dramatically, with great potential for clinic applications. The system consists of two key molecules: a CRISPR-associated (Cas) effector nuclease and a single guide RNA. The simplicity of the system has enabled the development of a wide spectrum of derivative methods. Almost any laboratory can utilize these methods, although new users may initially be confused when faced with the potentially overwhelming abundance of choices. Cas nucleases and their engineering have been systematically reviewed previously. In the present review, we discuss single guide RNA engineering and design strategies that facilitate more efficient, more specific and safer gene editing.
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Ma et al. (2021) conducted a review in Genome editing. Single guide RNA engineering and design strategies was evaluated. Single guide RNA engineering and design strategies facilitate more efficient, specific, and safer gene editing within the CRISPR/Cas system.
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