Conformational transition of regenerated Antheraea pernyi silk fibroin sponge treated with aqueous ethanol solution and in vitro wound healing effect of wild silk fibroin solution

작잠 누에고치를 정련한 후 질산칼슘4수화물의 용융액을 사용하여 재생 작잠 실크피브로인 스펀지를 제조하였다. 작잠 실크피브로인은 280 nm에서 tyrosine 잔기 등에 기인한 흡광대를 나타내었다. 작잠 실크피브로인 스펀지를 에탄올 농도별로 처리한 후 구조 전이를 관찰한 결과 80% 에탄올 처리시에는 β -sheet 구조( 700cm⁻¹ ), α -helix 구조( 625cm⁻¹ ), 그리고 random coil ( 660cm⁻¹ ) 구조가 공존하는 것으로 나타났다. 또한 작잠 실크피브로인을 이용한 in vitro 상처회복실험 결과 실크피브로인의 첨가에 의하여 상처회복 효과가 인정되었다. Regenerated Antheraea pernyi silk sponge was prepared using calcium nitrate 4 hydrate melt and examined the conformational changes treated with aqueous ethanol solution. The conformation of silk sponges was changed from random coil structure to β -sheet and α -helix conformation with low ethanol concentration (50 ~ 70%). On the other hand, that of silk sponges with 80% ethanol treatment showed β -sheet ( 700cm⁻¹ ), α -helix ( 625cm⁻¹ ), and random coil ( 660cm⁻¹ ) specific peaks. Wound healing effect in vitro was observed by cytoslective wound healing kit. Therefore, regenerated Antheraea pernyi silk sponges might be used as promising wound dressing materials.