The isomerization of D ‐glucose into D ‐fructose catalyzed by whole‐cell immobilized glucose isomerase. The dependence of the intrinsic rate of reaction on substrate concentration, pH, and temperature

The relation between the intrinsic rate of the glucose‐fructose isomerization catalyzed by whole‐cell immobilized glucose isomerase and the substrate concentration can be described with the kinetic model equation image The numerical values of k1, k−1, k2, and k−2 have been determined from low‐conversion experiments starting from pure glucose or fructose solutions, and are presented as a function of pH and temperature. The difference between the overall chemical reaction rate determined in high‐conversion experiments and that calculated from the individual k1, k−1, k2, and k−2 values is less than 10%.