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To assess the efficacy of current popular plastination dehydration techniques, a variety of organs were dehydrated to: 1. Determine the minimal length of time necessary to thoroughly dehydrate specimens for impregnation and 2. Measure tissue shrinkage during dehydration. Dehydrating agents commonly used for plastination (both room and cold temperature acetone and room temperature methanol) were evaluated. Cold acetone dehydration produced the least amount of tissue shrinkage. Shrinkage was greatest in the graded methanol series. Minimal length of time necessary for acetone dehydration was five days for both cold and room-temperature acetone dehydration.
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