Elucidation of rRNA Secondary Structure and Phylogenetic Analysis of Salmonella enterica based on 16S RNA

Salmonella enterica is a Gram-negative, facultatively anaerobic rod and a member of the Enterobacteriaceae. There are currently more than 2500 serovars of S. enterica. S. enterica is an important cause of foodborne illness in the developing countries and causes sporadic outbreaks of Salmonellosis in the developed countries. In the present study the isolate was confirmed on the basis of Gram staining and was subjected to various biochemical tests. Identification of bacterial strain was carried out using 16SrRNA gene sequencing. The isolate was found to be Salmonella enterica and most similar to Salmonella enterica subsp. enterica serovar Panama str. ATCC 7378. RNA secondary structure prediction has been performed. The 16S RNA gene sequence obtained was used to deduce the secondary structure of RNA ViennaRNA Web Services.