We investigated the relationship between the conserved electrophoretic pattern of nine double-stranded RNAs (dsRNAs) and the viruslike particles (VLPs) associated with La France disease of the button mushroom, Agaricus bisporus. Using a purification procedure involving chloroform extraction, PEG-NaCl precipitation, differential centrifugation, and equilibrium centrifugation in cesium-sulphate gradients, we have obtained preparations from diseased sporophores that were highly enriched in a 36-nm isometric VLP and contained minor amounts of both a 25-nm isometric VLP and the 19-×50-nm single-stranded RNA bacilliform virus (...)
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Michael M. Goodin (1992) studied this question.