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Cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase catalyzes the reversible formation of cytidine monophosphate 3-deoxy-D-manno-octulosonatefrom cytidine triphosphate and 3-deoxy-D-manno-octulosonate.The synthetase has been purified approximately 170-fold from crude extracts of Escherichia coli 0111-B 4 .The purified enzyme exhibits an absolute requirement for a divalent cation (Mg + + ) and is specific for cytidine triphosphate (Km = 2.2 X 10 -4 M) and 3-deoxy-D-manno-octulosonate (Km = 8 X 10 -4 M).
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Ghalambor et al. (1966) studied this question.
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