The hydrolysis of phenyl phosphate by mouse-liver acid phosphatase

To determine acid-phosphatase activities of liver homogenates, with phenyl phosphate as substrate, previous workers have usually adopted methods similar to the Robinson, Gutman & Gutman (1939) adaptation of the King & Arnstrong (1934) method for serum phosphatase (see, for example, Greenstein, 1942; Kochakian & Fox, 1944; Abul-Fadl & King, 1949; Goodlad & Mills, 1957). How- ever, results obtained by Tsuboi (1952), in studies of the hydrolysis of phenyl phosphate by mouse-liver acid phosphatase, have indicated that the assay conditions of the Robinson et al. (1939) method, and of similar methods, allow only partial expres- sion of the capacity of liver homogenate to hydro-