Chemical Rescue of c:\2020\08-aug-elbm0001-0142F508-CFTR Mimics Genetic Repair in Cystic Fibrosis Bronchial Epithelial Cells

In a previous study of sodium 4-phenylbutyrate (4-PBA)-responsive proteins in cystic fibrosis (CF) IB3-1 bronchial epithelial cells, we identified 85 differentially expressed high abundance proteins from whole cellular lysate (Singh, O. V., Vij, N., Mogayzel, P. J., Jr., Jozwik, C., Pollard, H. B., and Zeitlin, P. L. (2006) Pharmacoproteomics of 4-phenylbutyrate-treated IB3-1 cystic fibrosis bronchial epithelial cells. J. Proteome Res. 5, 562–571). In the present work we hypothesize that a subset of heat shock proteins that interact with cystic fibrosis transmembrane conductance regulator (CFTR) in common during chemical rescue and genetic repair will identify therapeutic networks from cellular and and with from and with proteins and identified of proteins in in common and the and of proteins in subset of and with the of in and with the of the that with a of proteins that therapeutic and and in In a previous study of sodium 4-phenylbutyrate (4-PBA)-responsive proteins in cystic fibrosis (CF) IB3-1 bronchial epithelial cells, we identified 85 differentially expressed high abundance proteins from whole cellular lysate (Singh, O. V., Vij, N., Mogayzel, P. J., Jr., Jozwik, C., Pollard, H. B., and Zeitlin, P. L. (2006) Pharmacoproteomics of 4-phenylbutyrate-treated IB3-1 cystic fibrosis bronchial epithelial cells. J. Proteome Res. 5, 562–571). In the present work we hypothesize that a subset of heat shock proteins that interact with cystic fibrosis transmembrane conductance regulator (CFTR) in common during chemical rescue and genetic repair will identify therapeutic networks from cellular and and with from and with proteins and identified of proteins in in common and the and of proteins in subset of and with the of in and with the of the that with a of proteins that therapeutic and and in fibrosis (CF) cystic cystic fibrosis transmembrane conductance sodium heat shock of cystic cystic fibrosis transmembrane conductance sodium heat shock of in the cystic fibrosis transmembrane conductance regulator (CFTR) and in the cystic fibrosis transmembrane conductance regulator (CFTR) a from the common and of the and conductance and proteins with a a regulator of sodium the a in the and and and in the a cellular that the chemical rescue sodium 4-phenylbutyrate of a of the the in In of with sodium 4-phenylbutyrate in cystic fibrosis epithelial and in of sodium 4-phenylbutyrate in cystic fibrosis of epithelial J. rescue with in a of heat shock proteins L. of 4-phenylbutyrate of IB3-1 bronchial epithelial a Jr., Pharmacoproteomics of 4-phenylbutyrate-treated IB3-1 cystic fibrosis bronchial epithelial Proteome Res. the chemical repair that in the of the of with during and from the the and that and of the the of of the in of the in cystic fibrosis the of the and of of the and the the networks of proteins during with a therapeutic with the the chemical repair during the rescue of the of genetic repair in the the of of the we the repair of the identified the proteins with in IB3-1 and with the that with in the of a subset of with and genetic repair and that a subset of proteins interact with therapeutic and the with a of and in and and and of J. common of cystic fibrosis transmembrane conductance regulator and in a P. the in of the cystic fibrosis transmembrane conductance regulator J. in J. of cystic fibrosis transmembrane conductance regulator in of from and the In of the a subset of with in IB3-1 with a the of H. interact with the cystic fibrosis transmembrane conductance J. 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