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A novel method was applied to improve the activity of enzymes immobilized in photocrosslinked poly(vinyl alcohol). Whereas the addition of glycerol to photosensitive poly(vinyl alcohol), bearing a styrylpyridinium group, diminishes the rate of photocrosslinking as well as the mechanical strength of the insolubilized gel, no considerable decrease in the rate of photoreaction was observed when poly(vinyl alcohol) was mixed with poly(ethylene glycol). This difference is based on the phase separation of the two water‐soluble polymers, resulting in the formation of a porous structure of the photocrosslinked gel. Immobilization of invertase in the photocrosslinked gel in the presence of poly(ethylene glycol) changes the activity, because of the case of diffusion of the substrate into the porous matrix.
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Kunihiro Ichimura (1987) studied this question.
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