Computer-assisted prediction of retention in multiple-development thin-layer chromatography

Retention of DNP derivatives of amino acids has been described by equations based on polynomial and Snyder-Soczewiński models. Models of retention were used to predict RF values in simulation of multiple development chromatography in different modes and the values calculated were compared with those obtained experimentally. Beside the retention of solutes in MD TLC, the widths of the spots were also predicted and compared with those obtained experimentally. Assessments of proposed models of multiple development were verified by experimental results and estimated by statistical testing.