The internal ATP concentration of Chara internodes was measured during metabolic poisoning. When the Chara was kept in the dark, the ATP level did not show any marked change at least for the first hour. Later it decreased slowly to less than half in about 4 days. The decrease in the ATP level was accelerated when the Chara was treated with 2 μM triphenyltin chloride (TPC), 0.2 mM 2,4-dinitrophenol (DNP), 1 mM monoiodoacetic acid (MIA) or 50 μM DCCD. The final decreased level of ATP in TPC or DNP was about 50% in the light, but less than 40% in the dark. The speed of the decrease in the ATP level depended on the concentration of these poisons. Additional application of 1 mM MIA lowered the final level to almost 10%. Simultaneous measurements of ADP and AMP concentrations showed a temporary increase of ADP and a later slow increase of AMP during poisoning.
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Takeuchi et al. (1983) studied this question.