Primary structure of elongation factor 1β from Artemia

cDNA complementary to mRNA coding for the elongation factor EF‐1β has been cloned. A γgt 11 cDNA library has been screened with an antiserum against EF‐1β which exchanges GDP bound to EF‐1α with exogenous GTP during protein synthesis. The derived amino acid sequence corresponds to 208 amino acids including the N‐terminal methionine which is absent in the mature protein. About sixty percent of the protein was sequenced by direct protein sequence analysis. Comparison of Artemis salina EF‐1β with Escherichia coli EF‐Ts shows no evident homology.