Some of these problems are now beliag studied by us, with interesting results.We had already been using this method for some time in the laboratory, when our attention was drawn to a paper by Paul Carnot and Louis Fournier,' who, independently and unknown to ourselves, have described a similar method for ebtaining the toxin produced by the pneumococcus in caltivating media.We are greatly indebted to Dr. Pinching Bey and Dr. Schiess Bey for allowing us to begin this work in the bacterio- togical laboratory of the Government hospital, Alexandria.To the latter gentleman we are especially indebted for much valuable assistance.REFzRENCE.
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Grassi et al. (1900) studied this question.