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Reference method for Total SugarsTotal sugars were quantified by HPLC (High performance liquid chromatography) through a methodology adapted from Kelebek et al. (2009), using a Varian liquid chromatograph equipped with a HPX 87H column and a refractive index (RI) detector.The column was maintained at room temperature (25 °C).Sulfuric acid (H 2 SO 4 0.5 mM) was
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Borba et al. (2017) studied this question.
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