Escherichia coli pgsA3 mutants deficient in acidic phospholipids were found to be defective in flagellar formation. This was first noticed by the unusual protein patterns of outer membrane fractions and was confirmed by measuring whole-cell total f1agellin. pgsA3 mutants were completely nonmotie. The defects were suppressed by a plasmid carrying the pgsA coding region. Flagellin levels and motility of null cis mutants that lacked cardiolipin synthase were almost normal. We conclude that the flagellum synthesis in E. coli is dependent on the membrane levels of acidic phospholipids.
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Nishino et al. (1993) studied this question.
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