The hypothesis that benomyl protection against ozone injury is due to the benzimidazole moiety was tested. Three concentrations of benzimidazole, benomyl, and Thiabendazole (2-(4-Thazolyl)-benzimidazole) were incorporated into a soil-peat-perlite (2:1:1) growth mixture. Plants grown in the treated soil mixtures were fumigated for 4 hr with 25 parts/hundred million (pphm) of ozone. Benzimidazole and benomyl protected the plants from ozone injury; Thiabendazole did not. A bioassay technique used in these studies indicated that failure of Thiabendazole to protect against ozone injury may have been caused by low uptake of the chemical. The technique involved measurement of zones of Penicillium cyclopium growth inhibition surrounding leaf discs placed on inoculated malt agar plates.
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M. Pellissier (1972) studied this question.