Chromatographic resolution of chiral intermediates in β‐adrenergic blocker synthesis on chiral stationary phases

The enantiomeric purities of optically active intermediates for beta-adrenergic blocking agents prepared via enzyme-assisted processes can be determined rapidly and with high accuracy using HPLC on commercially available columns with chiral supports [Chiralcel OD, OB; Chiralpak OT(+)]. The dependence of the resolution parameters on the substitution pattern of both hydroxy compounds and their esters is reported.