The Kinetics of Protein Denaturation

and Heinsius [1874] first pointed out that if a protein solution be dialysed until free from electrolytes then no coagulation occurs on boiling. If, however, a solution so treated is cooled and a little salt added in the cold, flocculation immediately takes place. From this it is inferred that although the process of denaturation can take place in the absence of salt, yet the salt, presumably as electrolyte, is necessary for the final stage in coagulation, viz. flocculation.