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Effect of changing energy levels of the diet at constant protein intakes on nitrogen balances in five young men were studied at two levels of protein--40 and 60 g. Nitrogen balance increased with increase in energy intake at both levels of protein. This was brought about primarily by a reduction in urinary nitrogen excretion. Energy levels at which nitrogen equilibrium was obtained were different for the two levels of protein intake. At 40 g protein intake the energy intake required for zero nitrogen balance was 2,249 kcal, while it was 2,066 kcal when the diet provided 60 g protein.
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Rao et al. (1975) studied this question.
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