Proliferative and antioxidant activity ofSymphytum officinaleroot extract

Ireneusz Sowaa, Roman Paduchbc, Maciej Strzemskia, Sylwia Zielińskad, Ewelina Rydzik-Strzemskaa, Jan Sawickia, Ryszard Kocjana, Janusz Polkowskia, Adam Matkowskid, Michał Latalskie & Magdalena Wójciak-Kosiora*a Department of Analytical Chemistry, Medical University of Lublin, Lublin, Polandb Faculty of Biology and Biotechnology, Department of Virology and Immunology, Maria Curie-Sklodowska University, Lublin, Polandc Department of General Ophthalmology, Medical University of Lublin, Lublin, Polandd Department of Pharmaceutical Biology, Wroclaw Medical University, Wroclaw, Polande Children’s Orthopedics Department, Medical University of Lublin, Lublin, Poland* Corresponding author. Email: kosiorma@wp.plAbstractThe root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC–DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts.