What does this research mean for the field?

A newly developed reverse-phase HPLC method provides a valid, reliable, and environmentally sustainable approach for the simultaneous quantification of Olmesartan Medoxomil and Azelnidipine, including under various stress conditions. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To develop and validate a stability-indicating, environmentally sustainable reverse-phase HPLC method for simultaneous quantification of olmesartan medoxomil and azelnidipine.

August 31, 2026Separation Science Plus

Development and Validation of Stability‐indicating Simultaneous Reverse‐Phase High‐Performance Liquid Chromatography Method for Olmesartan Medoxomil and Azelnidipine in Combination: A Computational Approach Toward Greenness

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Authors

MMMeera Maru KSKetan Shah

Discussion

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Overview

Laboratory validation study demonstrates an eco-friendly RP-HPLC assay for combined olmesartan medoxomil and azelnidipine, indicating reliable stability testing with minimal environmental impact.

Key Points

To develop and validate a stability-indicating, environmentally sustainable reverse-phase HPLC method for simultaneous quantification of olmesartan medoxomil and azelnidipine.
Separation was performed on a C18 column (150 mm × 4.6 mm, 5 µm) at room temperature using an isocratic mobile phase of 0.05 M ammonium formate buffer (pH 3.5) and methanol (30:70 %v/v) detected at 262 nm.
Method validation followed ICH guidelines across precision, accuracy, LOD, LOQ, robustness, and forced degradation under acidic, basic, oxidative, photolytic, and thermal stress.
Greenness assessment of the analytical workflow was computationally evaluated to quantify environmental sustainability relative to reported procedures.
Retention times were 3.71 min for olmesartan medoxomil and 5.39 min for azelnidipine, with linear detector responses across 10–30 µg/mL and 4–12 µg/mL, respectively (R² = 0.999 for both).
Mean recovery values ranged between 99.33% and 101.43%, with precision, LOD, LOQ, and robustness satisfying ICH acceptance criteria.
Forced degradation confirmed chromatographic resolution of both drugs from stress-induced degradation products alongside superior analytical greenness scores.

Cite This Study

Maru et al. (2026) studied this question.

synapsesocial.com/papers/6a95427bf20e493292a74da3 https://doi.org/10.1002/sscp.70294

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August 31, 2026Separation Science Plus

Development and Validation of Stability‐indicating Simultaneous Reverse‐Phase High‐Performance Liquid Chromatography Method for Olmesartan Medoxomil and Azelnidipine in Combination: A Computational Approach Toward Greenness

View Full Paper

Ask AI

Bookmark

Authors

MMMeera Maru KSKetan Shah

Discussion

Member takes

Overview

Laboratory validation study demonstrates an eco-friendly RP-HPLC assay for combined olmesartan medoxomil and azelnidipine, indicating reliable stability testing with minimal environmental impact.

Key Points

To develop and validate a stability-indicating, environmentally sustainable reverse-phase HPLC method for simultaneous quantification of olmesartan medoxomil and azelnidipine.
Separation was performed on a C18 column (150 mm × 4.6 mm, 5 µm) at room temperature using an isocratic mobile phase of 0.05 M ammonium formate buffer (pH 3.5) and methanol (30:70 %v/v) detected at 262 nm.
Method validation followed ICH guidelines across precision, accuracy, LOD, LOQ, robustness, and forced degradation under acidic, basic, oxidative, photolytic, and thermal stress.
Greenness assessment of the analytical workflow was computationally evaluated to quantify environmental sustainability relative to reported procedures.
Retention times were 3.71 min for olmesartan medoxomil and 5.39 min for azelnidipine, with linear detector responses across 10–30 µg/mL and 4–12 µg/mL, respectively (R² = 0.999 for both).
Mean recovery values ranged between 99.33% and 101.43%, with precision, LOD, LOQ, and robustness satisfying ICH acceptance criteria.
Forced degradation confirmed chromatographic resolution of both drugs from stress-induced degradation products alongside superior analytical greenness scores.

Cite This Study

Maru et al. (2026) studied this question.

synapsesocial.com/papers/6a95427bf20e493292a74da3 https://doi.org/10.1002/sscp.70294

View Full Paper

Ask AI

Bookmark