A substrate control system based upon dialysis culture techniques has been developed. In fermentations of Escherichia coli where phosphate or sulfate concentrations were controlled, the relation between the apparent specific growth rate, mu, and the phosphate concentration followed Monod kinetics, while the relation between mu and sulfate showed a sharp optimun. The pH of fermentations of E. coli was controlled by dialysis culture techniques without the fluctuations in pH associated with control by direct addition of acid or base.
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B. G. Thompson (1986) studied this question.
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