Key result
Recent advances in microfluidic devices and 3D printing have facilitated the development of sophisticated in-vitro thrombosis models to better reflect the in-vivo environment.
Why the study?
Models of arterial thrombus formation are vital tools to investigate platelet function and test novel antithrombotic drugs, with recent advances warranting a review of in vitro progress and future directions.
Recent advances in in-vitro flow chambers provide a more translational platform to test novel antithrombotics by better reflecting the in-vivo environment.
PURPOSE OF REVIEW: Models of arterial thrombus formation represent a vital experimental tool to investigate platelet function and test novel antithrombotic drugs. This review highlights some of the recent advances in modelling thrombus formation in vitro and suggests potential future directions. RECENT FINDINGS: Microfluidic devices and the availability of commercial chips in addition to enhanced accessibility of 3D printing has facilitated a rapid surge in the development of novel in-vitro thrombosis models. These include progression towards more sophisticated, 'vessel on a chip' models which incorporate vascular endothelial cells and smooth muscle cells. Other approaches include the addition of branches to the traditional single channel to yield an occlusive model; and developments in the adhesive coating of microfluidic chambers to better mimic the thrombogenic surface exposed following plaque rupture. Future developments in the drive to create more biologically relevant chambers could see a move towards the use of human placental vessels, perfused ex-vivo. However, further work is required to determine the feasibility and validity of this approach. SUMMARY: Recent advances in thrombus formation models have significantly improved the pathophysiological relevance of in-vitro flow chambers to better reflect the in-vivo environment and provide a more translational platform to test novel antithrombotics.
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Drysdale et al. (2023) conducted a review in arterial thrombus formation. in-vitro thrombosis models was evaluated. Recent advances in microfluidic devices and 3D printing have facilitated the development of sophisticated in-vitro thrombosis models to better reflect the in-vivo environment.
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