What question did this study set out to answer?

Determine whether dual infection of chick embryos with Melbourne and WSN influenza strains can yield stable combination viruses expressing antigenic traits from both parent lines.

July 1, 1953The Journal of Experimental MedicineOpen Access

The Experimental Production of Combination Forms of Virus

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Authors

GHGeorge K. HirstRockefeller Foundation TGTamar GotliebChildren's Hospital of Philadelphia

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Implication

Experimental study demonstrates the generation of stable hybrid influenza viruses in embryonated eggs, indicating antigenic recombination across viral strains.

Key Points

Determine whether dual infection of chick embryos with Melbourne and WSN influenza strains can yield stable combination viruses expressing antigenic traits from both parent lines.
Inoculated chick embryo allantoic sacs simultaneously with Melbourne (M) and WSN (W) influenza viruses.
Conducted serial passages in chorioallantoic sacs using standard inocula and limiting infective dilution series.
Characterized emerging strains via hemagglutinin inhibition and virus neutralization assays using parent-specific M and W antisera.
Initial hybrid X(1) required high inocula and rapidly reverted to parent strains, while successor form X(2) persisted through approximately 20 passages before segregating parental types.
A stable variant, X(3), emerged after 32 passages, propagating exclusively as X(3) without parental reversion across five serial limiting dilution passages.
X(3) hemagglutinin demonstrated strong inhibition by W antiserum and weak inhibition by M antiserum, while intact virus was significantly neutralized by both parental antisera.

Cite This Study

Hirst et al. (1953) studied this question.

synapsesocial.com/papers/6a9546d0abd4c05a5bf5977d https://doi.org/10.1084/jem.98.1.53

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Implication

Experimental study demonstrates the generation of stable hybrid influenza viruses in embryonated eggs, indicating antigenic recombination across viral strains.

The Experimental Production of Combination Forms of Virus

Discussion

Member takes

Implication

Key Points

Cite This Study

The Experimental Production of Combination Forms of Virus

Discussion

Member takes

Implication

Key Points

Cite This Study

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