Hydrolysis and Synthesis of Icosapentaenoic Acid-Docosahexaenoic Acid Rich Oil by Lipase TOYO (Chromobacterium viscosum)

The hydrolysis and synthesis of icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) rich oil by Lipase TOYO (Chromobacterium viscosum) were conducted.EPA and DHA were found to be released from the glycerol moiety at a constant rate, and were incorporated, by the reverse reaction, into the glycerol moiety using Lipase TOYO.The optimum water content and enzyme amount for synthesis of triglyceride were 0.93% and 200 units respectively. Under these conditions, using pure EPA as well as DHA as the substrate, 91. 3% of EPA and 94. 9% of DHA were incorporated into the glycerol moiety.