Conservation of the 3'-Terminal Sequences of the Genome RNA Species of Tomato Black Ring Virus

SUMMARY Sequences of more than 100 residues adjacent to the 3′-terminal poly(A) tract were determined for each of the two genome RNA species of tomato black ring nepovirus (TBRV) strains A and C; in addition a shorter portion of sequence was determined for RNA-2 of strain G. Comparison of these results and the sequence previously determined for RNA-2 of strain S showed that in this region the sequences of RNA-1 and RNA-2 are almost identical and that considerable sequence homology exists between strains. In contrast, a segment of sequence near the 3′ end of RNA-2 of tobacco ringspot virus, another nepovirus, showed no similarity with the corresponding region of TBRV RNA.