Human milk samples were divided at collection and stored at -70 degrees C or -20 degrees C, or extracted immediately with organic solvent, to compare lipid class composition. Storage at -20 degrees C was not satisfactory for maintaining milk lipid composition, for it resulted in hydrolysis of triglycerides and the appearance of free fatty acids.
No takes yet. Share an insight, caveat, or question.
Bitman et al. (1983) studied this question.