Enzyme-Catalyzed Azepinoindole Formation in Clavine Alkaloid Biosynthesis

This is the authors' abstract. We don't add key points for this paper.

(−)-Aurantioclavine ( 1 ), which contains a characteristic seven-membered ring fused to an indole ring, belongs to the azepinoindole class of fungal clavine alkaloids. Here we show that starting from a 4-dimethylallyl- l -tryptophan precursor, a flavin adenine dinucleotide (FAD)-binding oxidase and a catalase-like heme-containing protein are involved in the biosynthesis of 1 . The function of these two enzymes was characterized by heterologous expression, in vitro characterization, and deuterium labeling experiments.