The cell-surface affinity matrix technology, otherwise called "secretion assay" or "capture assay," represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen-specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.
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Assenmacher et al. (2001) studied this question.
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