Inducible β‐Xyloside Permease as a Constituent of the Xylan‐Degrading Enzyme System of the Yeast Cryptococcus albidus

The yeast, Cryptococcus albidus, depending on whether it is grown on xylan or glucose, differs remarkably in the ability to take up inducers of extracellular endo-1,4-beta-xylanase synthesis. In washed, glucose-grown cells the initially low ability to take up xylobiose or methyl beta-D-xylopyranoside, increases during incubation with these compounds after a lag-phase shorter than the induction time of the extracellular beta-xylanase. Using of methyl beta-D-[U-14C]xylopyranoside as a very slowly metabolizable inducer of beta-xylanase it has been established that the increase of the rate of xylobiose or methyl xyloside uptake is due to induction of an active transport system for methyl beta-D-xyloside and beta-1,4-xylooligosaccharides. The system is called beta-xyloside permease. The permease activity of induced cells decreases in the absence of beta-xylanase inducers. The induction of permease as well as its inactivation (degradation) can be prevented with cycloheximide, thus both events appear to be dependent on de novo protein synthesis. In analogy with other active transport systems, beta-xyloside permease function can be effectively blocked by inhibitors of energy metabolism in the cells. The demonstrated example of induction of a permease, for inducers and products of hydrolysis of an extracellular polysaccharide hydrolase, points to a new feature of induction of extracellular enzymes in eucaryotic microorganisms.